{"id":15556,"date":"2020-10-21T12:06:14","date_gmt":"2020-10-21T09:06:14","guid":{"rendered":"https:\/\/kanidis.gr\/?page_id=15556"},"modified":"2024-02-15T15:53:52","modified_gmt":"2024-02-15T12:53:52","slug":"immunohistochemistry-in-situ","status":"publish","type":"page","link":"https:\/\/kanidis.gr\/en\/immunohistochemistry-in-situ\/","title":{"rendered":"Immunohistochemistry > In Situ"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/6&#8243;][vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"kanidis-product alignleft wp-image-15394 \" src=\"https:\/\/kanidis.gr\/wp-content\/uploads\/hybridizer.jpg\" alt=\"Hybridizer\" width=\"222\" height=\"173\" \/>[\/vc_column_text][\/vc_column][vc_column width=&#8221;4\/6&#8243;]<div id=\"ultimate-heading-68646a713c909e0d5\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-68646a713c909e0d5 uvc-2601  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"left\" style=\"text-align:left\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-68646a713c909e0d5 h3'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h3 style=\"--font-weight:theme;margin-bottom:20px;\">Hybridizer<\/h3><\/div><\/div>[vc_column_text css=&#8221;.vc_custom_1708000456338{padding-top: 20px !important;}&#8221;]\u0395\u03b9\u03b4\u03b9\u03ba\u03ae \u03c3\u03c5\u03c3\u03ba\u03b5\u03c5\u03ae \u03b3\u03b9\u03b1 \u03b5\u03c6\u03b1\u03c1\u03bc\u03bf\u03b3\u03ad\u03c2 In Situ \u03c5\u03b2\u03c1\u03b9\u03b4\u03b9\u03c3\u03bc\u03bf\u03cd \u03ba\u03b1\u03b9 \u03bc\u03b5\u03b8\u03cc\u03b4\u03bf\u03c5\u03c2 FISH &amp; CISH.<\/p>\n<ul>\n<li>\u03a7\u03c9\u03c1\u03b7\u03c4\u03b9\u03ba\u03cc\u03c4\u03b7\u03c4\u03b1 12 \u03b1\u03bd\u03c4\u03b9\u03ba\u03b5\u03b9\u03bc\u03b5\u03bd\u03bf\u03c6\u03cc\u03c1\u03b5\u03c2 \u03c0\u03bb\u03ac\u03ba\u03b5\u03c2<\/li>\n<li>\u0395\u03cd\u03c1\u03bf\u03c2 \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1\u03c2 \u03b1\u03c0\u03cc 50<sup>\u03bf<\/sup>\u00a0\u03c9\u03c2 99<sup>\u03bf<\/sup>\u00a0C<\/li>\n<li>\u03a7\u03c1\u03cc\u03bd\u03bf\u03c2 \u03b1\u03cd\u03be\u03b7\u03c3\u03b7\u03c2 \u03c4\u03b7\u03c2 \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1\u03c2 \u03b1\u03c0\u03cc 37<sup>\u03bf<\/sup>\u00a0\u03c3\u03b5 95<sup>\u03bf<\/sup>\u00a0C \u03bc\u03cc\u03bd\u03bf 2 \u03bb\u03b5\u03c0\u03c4\u03ac<\/li>\n<li>\u0391\u03bd\u03c4\u03af\u03c3\u03c4\u03bf\u03b9\u03c7\u03bf\u03c2 \u03c7\u03c1\u03cc\u03bd\u03bf\u03c2 \u03c8\u03cd\u03be\u03b7\u03c2 \u03c3\u03b5 5 \u03bb\u03b5\u03c0\u03c4\u03ac<\/li>\n<li>\u0394\u03c5\u03bd\u03b1\u03c4\u03cc\u03c4\u03b7\u03c4\u03b1 \u03b1\u03c0\u03bf\u03b8\u03ae\u03ba\u03b5\u03c5\u03c3\u03b7\u03c2 40 \u03c0\u03c1\u03bf\u03b3\u03c1\u03b1\u03bc\u03bc\u03ac\u03c4\u03c9\u03bd<\/li>\n<li>\u0394\u03c5\u03bd\u03b1\u03c4\u03cc\u03c4\u03b7\u03c4\u03b1 \u03b5\u03c0\u03ce\u03b1\u03c3\u03b7\u03c2 \u03c3\u03b5 \u03c3\u03c4\u03b1\u03b8\u03b5\u03c1\u03ae \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1<\/li>\n<li>\u03a3\u03c4\u03b1\u03b8\u03b5\u03c1\u03cc\u03c4\u03b7\u03c4\u03b1 \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1\u03c2\u00a0 +\/- 1<sup>\u03bf<\/sup>\u00a0C<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/6&#8243;]<div id=\"ultimate-heading-60076a713c909e19c\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-60076a713c909e19c uvc-8140 pdf uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"left\" style=\"text-align:left\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-sub-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-60076a713c909e19c .uvc-sub-heading '  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"font-weight:normal;\"><strong><a href=\"https:\/\/kanidis.gr\/wp-content\/uploads\/hybridizer.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Hybridizer<\/a><\/strong><\/div><\/div>[vc_separator color=&#8221;white&#8221; css=&#8221;.vc_custom_1706609592736{margin-top: 15px !important;margin-bottom: 5px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;peacoc&#8221; css=&#8221;.vc_custom_1602852718815{margin-top: 30px !important;margin-bottom: 30px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/www.agilent.com\/cs\/publishingimages\/1059834.jpg\" alt=\"HER2 IQFISH pharmDx\" width=\"170\" height=\"170\" \/>[\/vc_column_text][\/vc_column][vc_column width=&#8221;4\/6&#8243;]<div id=\"ultimate-heading-51566a713c909e247\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-51566a713c909e247 uvc-4851  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"left\" style=\"text-align:left\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-51566a713c909e247 h3'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h3 style=\"--font-weight:theme;\">HER2 IQFISH pharmDx<\/h3><\/div><\/div>[vc_column_text]<i>HER2<\/i>\u00a0IQFISH pharmDx is a direct fluorescence in situ hybridization (FISH) assay based on Dako&#8217;s new fast IQISH hybridization buffer chemistry. The IQISH hybridization buffer is non-toxic and allows genomic DNA probe hybridization to be performed in just 60-120 minutes. The short hybridization time results in a turnaround time of less than 4 hours for a complete FISH staining from deparaffinization to mounting.<\/p>\n<p><i>HER2<\/i>\u00a0IQFISH pharmDx is designed to quantitatively determine\u00a0<i>HER2<\/i>\u00a0gene amplification in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue specimens and FFPE specimens from patients with adenocarcinoma of the stomach, including gastroesophageal junction.\u00a0<i>HER2<\/i>\u00a0IQFISH pharmDx with the indication adenocarcinoma of the stomach, including the gastroesophageal junction, is not available in all markets. Gene amplification is determined from the ratio between the number of signals from the hybridization of the\u00a0<i>HER2<\/i>\u00a0gene probe (red signals) and the number of signals from the hybridization of the CEN-17 reference chromosome 17 probe (green signals).[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/6&#8243;]<div id=\"ultimate-heading-99496a713c909e2ea\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-99496a713c909e2ea uvc-2776 pdf uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-sub-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-99496a713c909e2ea .uvc-sub-heading '  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"font-weight:normal;\"><strong><a href=\"http:\/\/www.agilent.com\/cs\/library\/packageinsert\/public\/Copy%20of%20PD04090EFG_04.pdf\" target=\"_blank\" rel=\"noopener\">HER2 IQFISH<\/a><\/strong><\/div><\/div>[vc_separator color=&#8221;white&#8221; css=&#8221;.vc_custom_1706609592736{margin-top: 15px !important;margin-bottom: 5px !important;}&#8221;][vc_column_text]\u2611 20 Test<\/p>\n<p><span class=\"yKMVIe\" role=\"heading\" aria-level=\"1\">\u2611 IVDR<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;peacoc&#8221; css=&#8221;.vc_custom_1602852718815{margin-top: 30px !important;margin-bottom: 30px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][vc_single_image image=&#8221;15808&#8243; img_size=&#8221;medium&#8221; onclick=&#8221;custom_link&#8221; link=&#8221; https:\/\/kanidis.gr\/en\/immunohistochemistry-in-situ-1\/&#8221;][\/vc_column][vc_column width=&#8221;4\/6&#8243;]<div id=\"ultimate-heading-87946a713c909e391\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-87946a713c909e391 uvc-5348  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"left\" style=\"text-align:left\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-87946a713c909e391 h3'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h3 style=\"--font-weight:theme;\">IQFISH Panel for Lung Cancer<\/h3><\/div><\/div>[vc_column_text]The IQFISH panels include a set of oligonucleotide-based ready-to-use FISH probes, premixed with IQFISH hybridization buffer, for the detection of gene rearrangements and for the detection of gene amplifications by fluorescence in situ hybridization (FISH) on formalin-fixed, paraffin-embedded (FFPE) tissue sections. The probes consist of two child probes (3\u2019 and 5\u2019 probes), each designed to target a given gene and each labeled in a different color (orange-red Cy3 and green FITC).<\/p>\n<p>The assays combine two ground-breaking technologies: oligonucleotide-based SureFISH technology and the formamide-free IQFISH hybridization buffer. SureFISH technology enables chromosomal aberration detection using a precise, synthetic oligonucleotide probe design process. IQFISH hybridization buffer is a unique, ultra-fast hybridization technology that allows probe hybridization to be performed in just 1-2 hours and provides complete FISH results in about 4 hours. Together these advancements provide fast, high-quality FISH results.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/6&#8243;][vc_separator color=&#8221;white&#8221; css=&#8221;.vc_custom_1706609592736{margin-top: 15px !important;margin-bottom: 5px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;peacoc&#8221; css=&#8221;.vc_custom_1602852718815{margin-top: 30px !important;margin-bottom: 30px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/www.ngaio.co.nz\/wp-content\/uploads\/2018\/05\/Image_K5799-300x300.jpg\" alt=\"Histology FISH Accessory Kit - Ngaio Diagnostics\" width=\"170\" height=\"170\" \/>[\/vc_column_text][\/vc_column][vc_column width=&#8221;4\/6&#8243;]<div id=\"ultimate-heading-47746a713c909e437\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-47746a713c909e437 uvc-6753  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"left\" style=\"text-align:left\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-47746a713c909e437 h3'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h3 style=\"--font-weight:theme;\">IQFISH Fast Hybridization Buffer<\/h3><\/div><\/div>[vc_column_text]IQFISH Fast Hybridization Buffer is a formamide-free hybridization buffer that enables ISH hybridization to be completed in 1-1.5 hours. A complete in situ hybridization (ISH) assay on a formalin-fixed, paraffin-embedded (FFPE) sample can be completed in 4 hours enabling a half day sample-to-result workflow. This workflow offers many operation advantages such as increased turnaround time, same day repeat testing without impacting the quality of hybridization.<\/p>\n<p>Standard formamide-based FISH hybridization buffer lowers the melting temperature of nucleic acids by targeting hydrogen bonds and interfering with DNA base pairing resulting in the denaturation of the DNA double strand. The interference slows down the following base pairing between the FISH probe and specimen DNA. However, IQFISH buffer destabilizes the DNA helix by diminishing hydrophobic base stacking (1). Minimized based paring interference enables fast binding between the ISH probe and specimen DNA.<\/p>\n<p>IQFISH buffer is provided in three different size formats to meet the needs of varying testing volumes.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/6&#8243;]<div id=\"ultimate-heading-59346a713c909e4d9\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-59346a713c909e4d9 uvc-8260 pdf uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-sub-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-59346a713c909e4d9 .uvc-sub-heading '  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"font-weight:normal;\"><a href=\"https:\/\/www.agilent.com\/cs\/library\/usermanuals\/public\/5991-9878.pdf\" target=\"_blank\" rel=\"noopener\"><b>IQFISH Buffer<\/b><\/a><\/div><\/div>[vc_separator color=&#8221;white&#8221; css=&#8221;.vc_custom_1706609592736{margin-top: 15px !important;margin-bottom: 5px !important;}&#8221;][vc_column_text]\u2611 900 \u03bcL<\/p>\n<p><span class=\"yKMVIe\" role=\"heading\" aria-level=\"1\">\u2611 IVDR<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;peacoc&#8221; css=&#8221;.vc_custom_1602852718815{margin-top: 30px !important;margin-bottom: 30px !important;}&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/6&#8243;][vc_column_text][\/vc_column_text][\/vc_column][vc_column width=&#8221;4\/6&#8243;][vc_column_text css=&#8221;.vc_custom_1708000456338{padding-top: 20px !important;}&#8221;]\u0395\u03b9\u03b4\u03b9\u03ba\u03ae \u03c3\u03c5\u03c3\u03ba\u03b5\u03c5\u03ae \u03b3\u03b9\u03b1 \u03b5\u03c6\u03b1\u03c1\u03bc\u03bf\u03b3\u03ad\u03c2 In Situ \u03c5\u03b2\u03c1\u03b9\u03b4\u03b9\u03c3\u03bc\u03bf\u03cd \u03ba\u03b1\u03b9 \u03bc\u03b5\u03b8\u03cc\u03b4\u03bf\u03c5\u03c2 FISH &amp; CISH. \u03a7\u03c9\u03c1\u03b7\u03c4\u03b9\u03ba\u03cc\u03c4\u03b7\u03c4\u03b1 12 \u03b1\u03bd\u03c4\u03b9\u03ba\u03b5\u03b9\u03bc\u03b5\u03bd\u03bf\u03c6\u03cc\u03c1\u03b5\u03c2 \u03c0\u03bb\u03ac\u03ba\u03b5\u03c2 \u0395\u03cd\u03c1\u03bf\u03c2 \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1\u03c2 \u03b1\u03c0\u03cc 50\u03bf\u00a0\u03c9\u03c2 99\u03bf\u00a0C \u03a7\u03c1\u03cc\u03bd\u03bf\u03c2 \u03b1\u03cd\u03be\u03b7\u03c3\u03b7\u03c2 \u03c4\u03b7\u03c2 \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1\u03c2 \u03b1\u03c0\u03cc 37\u03bf\u00a0\u03c3\u03b5 95\u03bf\u00a0C \u03bc\u03cc\u03bd\u03bf 2 \u03bb\u03b5\u03c0\u03c4\u03ac \u0391\u03bd\u03c4\u03af\u03c3\u03c4\u03bf\u03b9\u03c7\u03bf\u03c2 \u03c7\u03c1\u03cc\u03bd\u03bf\u03c2 \u03c8\u03cd\u03be\u03b7\u03c2 \u03c3\u03b5 5 \u03bb\u03b5\u03c0\u03c4\u03ac \u0394\u03c5\u03bd\u03b1\u03c4\u03cc\u03c4\u03b7\u03c4\u03b1 \u03b1\u03c0\u03bf\u03b8\u03ae\u03ba\u03b5\u03c5\u03c3\u03b7\u03c2 40 \u03c0\u03c1\u03bf\u03b3\u03c1\u03b1\u03bc\u03bc\u03ac\u03c4\u03c9\u03bd \u0394\u03c5\u03bd\u03b1\u03c4\u03cc\u03c4\u03b7\u03c4\u03b1 \u03b5\u03c0\u03ce\u03b1\u03c3\u03b7\u03c2 \u03c3\u03b5 \u03c3\u03c4\u03b1\u03b8\u03b5\u03c1\u03ae \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1 \u03a3\u03c4\u03b1\u03b8\u03b5\u03c1\u03cc\u03c4\u03b7\u03c4\u03b1 \u03b8\u03b5\u03c1\u03bc\u03bf\u03ba\u03c1\u03b1\u03c3\u03af\u03b1\u03c2\u00a0 +\/- 1\u03bf\u00a0C&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/pages\/15556"}],"collection":[{"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/comments?post=15556"}],"version-history":[{"count":5,"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/pages\/15556\/revisions"}],"predecessor-version":[{"id":15817,"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/pages\/15556\/revisions\/15817"}],"wp:attachment":[{"href":"https:\/\/kanidis.gr\/en\/wp-json\/wp\/v2\/media?parent=15556"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}